Immune Stress and Reproduction: Insights Into the Role of Norepinephrine and GABA.

Immune Stress and Reproduction: Insights Into the Role of Norepinephrine and GABA.

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Immune stress compromises reproduction. The cytokine, interleukin-1beta (IL-1beta, an immune stressor has been has been shown to activate the stress axis and compromise the reproductive axis, resulting in loss of ovulation. The mechanisms behind these effects are still under investigation. The present work addresses the role of stimulatory neurotransmitters like norepinephrine (NE) and inhibitory ones like GABA in mediating IL-1beta's effects on reproduction. We hypothesized that systemic IL-1beta modulates the levels of NE either at the hypothalamus or brainstem and that GABA too might play a role in this phenomenon. The results of these studies revealed important and novel insights into neuroendocrine changes during a systemic immune challenge: (1) IL-1beta decreases NE input at the level of hypothalamus to inhibit LH, which could be reversed by NE precursor, L-dopa; (2) This decrease in NE was accompanied by increase in GABA and GABA-B antagonist could reverse the inhibitory effect of IL-1beta; (3)Simultaneous activation of the stress and reproductive axes in female rats by IL-1beta involves changes in NE levels in hypothalamus; (4)This could be due to changes in IL-1beta signaling at the cell bodies in the brainstem NE neurons. Together these findings provide strong evidence substantiating the role of NE in mediating the effects of a systemic immune challenge on reproduction and opens up exciting future research possibilities.I.Quantitative RT-PCR: RNA extraction: The RNA was extracted from the brainstem punches using MELT Total Nucleic Acid Isolation ... Superscript III First strand synthesis Supermix for qRT-PCR (Invitrogen, Carlsbad, CA) according to the manufacturera#39;s instructions. ... The reactions were performed in The Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems) with the following 47 G Hanbsp;...


Title:Immune Stress and Reproduction: Insights Into the Role of Norepinephrine and GABA.
Author:
Publisher:ProQuest - 2008
ISBN-13:

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